Molecular Diagnosis of Necrotizing Fasciitis by 16S rRNA Gene Sequencing and Superantigen Gene Detection
نویسندگان
چکیده
منابع مشابه
Molecular diagnosis of Kingella kingae pericarditis by amplification and sequencing of the 16S rRNA gene.
Kingella kingae is a fastidious gram-negative bacillus that is considered an emerging pathogen in pediatric settings but remains less common in adults. Here we describe a case of pericarditis in an immunocompetent adult host. The microorganism was identified directly from the clinical sample by molecular techniques, i.e., 16S rRNA gene amplification and sequencing.
متن کاملDETECTION OF BACTERIA BY AMPLIFYING THE 16S rRNA GENE WITH UNIVERSAL PRIMERS AND RFLP
Background: There is a conserved portion in the 16S rRNA gene of bacteria which can be amplified by the universal PCR method. This fragment is 996 bp in length. In this method, only one set of universal primers is used for the amplification of the conserved region of the 16S rRNA gene, in common bacterial pathogens. Therefore, using the universal PCR method, these bacteria are detectable on...
متن کاملDiversity of caprine and ovine Pasteurella multocida isolates based on 16S rRNA gene sequencing
In this study, to increase information about the relationship between caprine and ovine isolates of Pasteurella multocida, 16S rRNA gene sequencing of 9 goats (5) and sheep (4) isolates were investigated. Also, capsular type and toxAgene presentation was studied in this paper. All isolates, except one, belong to capsular type A, and toxA+ strain distributed among strains were isolated from both...
متن کاملPhylogenetic inference of Coxiella burnetii by 16S rRNA gene sequencing
Coxiella burnetii is a human pathogen that causes the serious zoonotic disease Q fever. It is ubiquitous in the environment and due to its wide host range, long-range dispersal potential and classification as a bioterrorism agent, this microorganism is considered an HHS Select Agent. In the event of an outbreak or intentional release, laboratory strain typing methods can contribute to epidemiol...
متن کاملSpecies-specific identification of Leptospiraceae by 16S rRNA gene sequencing.
The genus Leptospira is classified into 13 named species and 4 genomospecies based upon DNA-DNA reassociation studies. Phenotypic tests are unable to distinguish between species of Leptospira, and there is a need for a simplified molecular approach to the identification of leptospires. 16S rRNA gene sequences are potentially useful for species identification of Leptospira, but there are a large...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: The Journal of Molecular Diagnostics
سال: 2005
ISSN: 1525-1578
DOI: 10.1016/s1525-1578(10)60599-5